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KMID : 0383919700070000307
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Report of National Institute of Health
1970 Volume.7 No. 0 p.307 ~ p.320
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A Study on the determination of Paragontmus antibody in sera of infected animals and man by some immunoserological methods
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Abstract
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Immunoserological studies of paragonimiasis westermani were conducted in animals of experimental infection, i.e., white rats, hamsters and dogs, as well as in the sera of human paragonimiasis using immunoelectrophoresis, Ouchterlony test and complement fixation test. 0.1% NaCl antigen prepared from adult worms were used in immunoelectrophoresis and Ouchterlony test, while in complement fixation test VBS antigen was used. The antisera of the infected animals were preared serially in weekly intervals. The results are summarized as follows.
1. In white rats, by immunoelectrophoresis, the precipitin bands started to appear in 1-2 weeks after the infection and the number of precipitin bands were gererally 2-6 with the high of 9 until the 35 weeks after the infection when the animals were sacrificed. By Ouchterlony test, the precipitin bands appeared form 1-4 weeks after the infection and the number of precipitin bands was usually 1-3 with the high of 5. No difference was seen in the number of precipitin bands by the intensity of infection both in immunoelectrophoresis and Ouchterlony test. By complement fixation test, no reaction was seen generally in the sera of the rats.
2. In hamsters, by immunoelectrophoresis, the precipitin bands apeared in 3-5 week after the infection with the usual number of the bands 4-7 with the high of 12. In Ouchterlony test, the precipitin bands appeared in 2-4 weeks showing the number of the bands usually 2-4 with the high of 5. In complement fixation test, the reaction of the sera converted to positive 4-5 weeks after the infection.
3. In sera of dogs, all the tests, i.e., immunoelectrophoresis, Ouchterlony and complement fixation test showed irregular and unreliable reaction.
4. In sera of humman paragonimiasis, the above three techniques showed reliable reaction. From the results obtained in ithe tests of the antisera serially prepared from the animals, these tests showed the applicability in the diagnosis of the early stage of the infection in human paragonimiasis.
5. Rats and hamsters were unfavorable hosts of P. westermani, allowing the worms remain immature stage in the various part of the host, while dogs were favorable with the infection showing full development of the worms.
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